Library Preparation Workflows
- Standardized amplicon workflows (e.g., 16S approaches)
- Validated primer/adaptor resources
- Pre-plated primer systems
- Infrastructure suitable for low-input and environmental samples
All laboratory work is performed by trained users.
Quantification
- Qubit 4 Fluorometer
- BioTek Synergy Plate Reader
- Quant-iT assay kits available via recharge
Quality Control
- Agarose gel electrophoresis
- UVP Chemidoc imaging
- Agilent Bioanalyzer (users provide kits)
PCR & qPCR
- Thermocyclers (including gradient capability)
- ABI StepOne Real-Time PCR
- Bio-Rad CFX96 Real-Time PCR
Sample Preparation
- TissueLyser II
- Chemical fume hood for DNA/RNA extraction
- UV crosslinker
*GTAC provides infrastructure and training to support users in generating sequencing-ready libraries but does not perform sequencing.
GTAC Hours
Monday – Friday | 9:00 AM – 5:00 PM
After-hours access requires prior approval.
GTAC Open Lab Support Hours – HH125
In-person lab support with the GTAC Director for training, troubleshooting, and active research projects.
Monday | 11:00 AM – 1:00 PM
Thursday | 10:00 AM – 12:00 PM
No appointment required. Scheduled meetings welcome during this time.
Contact:
GTAC Director: Colleen M. Ingram, PhD
Ph: 415-338-6911
cingram@sfsu.edu